Solubilization of dopamine D-1 receptors with a zwitterionic detergent DCHAPS and their reconstitution.

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Summary

DCHAPS was the best solubilizer among all detergents used and at 0.075% DCHAPS, 10 mg/ml protein, 30 min, 4 degrees C, gave the yield of 48.7% suggesting that D CHAPS solubILized sufficient amounts of the membrane phospholipids.

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article
Published
1992-04-01
Cited by
2
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26

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